Iowa State Livestock Research Aims to Uncover How Biological Factors Impact Human Fertility

Thu, 17 Apr 2025 21:25:43 GMT

Despite having sperm with good motility (forward movement) and morphology (cell shape), one out of every four boars experience a conception rate of less than 80%. The issue mirrors human fertility challenges with one out of eight couples struggling with infertility, and one-third of those cases are attributed to male factors, according to the National Institute of Health.

“Pigs and cattle are species we have plenty of oocytes and sperm cells available for, allowing us to conduct in vitro fertilization research,” says Karl Kerns, Iowa State University assistant professor of animal science in a recent article . “They also are effective models for human reproduction compared to rodents, who do not model the human as well genetically or phenotypically at the cellular level.”

He dedicates his research efforts to pigs and cattle .

Kerns’ Lab specializes in molecular reproductive physiology with a focus on understanding and enhancing livestock sire fertility. The lab is equipped with state-of-the-art facilities, including andrology and molecular biology labs as well as an in vitro fertilization (IVF) lab. The IVF lab has computer-assisted semen analysis and time-lapse fluorescent microscopes to image-based flow cytometry paired with deep learning, artificial intelligence analysis methods and sorting flow cytometry.

The lab’s goal is to enhance reproductive efficiency by analyzing sperm quality and identifying biological markers that influence fertility rates. The process involves sorting sperm cells using advanced flow cytometry technology. The sorter system uses microfluidics and lasers to analyze and sort cells based on fluorescence-marked biomarkers.

“We can track thousands of cells per second, identifying key fertility indicators and sorting them accordingly,” Kerns says.

The process allows for an in-depth analysis of fertility potential and production capabilities.

Kerns’ team employs both positive and negative biomarkers to evaluate sperm quality. Positive biomarkers are generally considered good and reflect increased chances of conception, while negative biomarkers can indicate compromised fertility potential.

The team also assesses mitochondrial activity, zinc signatures and protein detection, all of which assist the lab in detecting and analyzing both healthy and unhealthy sperm.

Ian Shofner, a third-year doctorate student in mammalian reproductive physiology, focuses on artificial intelligence (AI) and developing models that predict sperm fertility metrics in the Kerns Lab.

“Currently, male fertility diagnostics are lacking in accuracy, and my work aims to improve these predictions for better assessments of male fertility in mammals,” Shofner explains. “With this, we intend on enabling the industry to make cost-effective AI solutions for fertility diagnostics using affordable brightfield microscopy technology.”

Tyler Weide, a third-year doctorate student in the interdepartmental genetics and genomics program, has an emphasis on uncovering the molecular and cellular mechanisms for fertilization.

“It’s an ideal environment for those interested in reproductive physiology to apply critical thinking in solving complex problems relevant to both agricultural and biomedical sciences,” Weide says.

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How Does Iowa State Research on Swine Sire Fertility Impact Cattle, Humans?

Wed, 23 Mar 2022 19:43:03 GMT

Sire infertility is a problem for the reproductive performance of swine, despite the greater attention that has long been given to the female side of the equation.

“We know that 25% of the boar herd has less than 80% conception rates, which is considered unacceptable by industry,” said Karl Kerns, assistant professor of animal science at Iowa State University and 2014 alum of the same department. “By adding even one more pig to a litter, we could increase production by an estimated $120 million annually. To improve that, we need to learn more about what influences the capacity of sperm to fertilize. That means developing better tools for researching sperm health – and making it easier for the industry to use the knowledge we already have.”

Kerns is leading a new five-year grant from the USDA National Institute for Food and Agriculture to support research on boar sperm capacitation -- the biological pathways that support spermatozoa’s ability to fertilize. The grant is one of 14 funded nationwide under NIFA’s Agriculture and Food Research Initiative (AFRI) - Animal Reproduction Program. This project, and two other recent awards to collaborate with colleagues at the University of Missouri and the USDA Agricultural Research Service, total $1.7 million in federal support for Kerns’ work studying male fertility.

Kerns takes a molecular approach to analyze the biochemical makeup of sperm, especially the proteins, fats and energy sources that signal which sperm are more – or less -- fertile. The AFRI project focuses on swine, but has implications for other species, including cattle and humans.

To study sire fertility, he uses a state-of-the-art piece of equipment found in few andrology labs around the world: an image-based flow cytometer, “basically a high-throughput microscope,” he explained. In a matter of a 30-60 seconds, the cytometer allows imaging of up to 10,000 sperm cells and up to nine biomarkers within each cell. Kerns then uses computer-based artificial intelligence to link the resulting vast data set of images with reproductive outcomes. This kind of equipment and approach has become more common in human health research, such as the cancer field, but it has not been used to study livestock fertility, according to Kerns.

The project has multiple goals, including reducing barriers in the swine industry to use the best available information on swine genetics and reproductive capacity.

“In the past, the uptake of relevant technology has been slowed by the high cost of lab equipment, the need for highly skilled labor and increased analysis time, none of which are conducive to production,” said Kerns. “This new project will use analytical software resources aimed at providing accessible, economical methods and equipment that can more quickly translate findings from the lab bench to barns.”

Another aspect of the research is to investigate the lipid (or fats), protein and energy sources of fertile spermatozoa to see if supplements can increase sperm cell survival after insemination and boost fertility.

His previous foundational work has already had an impact on the livestock industry. While doing graduate and postdoctoral research at the University of Missouri, he and his team found a relationship between zinc ions and the fertilization competency of boar spermatozoa. That information, now termed the “mammalian sperm zinc signature,” has improved boar and bull fertility diagnostics. Further research on the influence of zinc on sperm health is an ongoing area of focus.

“Male fertility is one part of the fertility equation that is often overlooked, but it’s critically important,” Kerns said. “However, this is not just about swine. We are taking a ‘One Health’ approach that is likely to be relevant to other mammals, including cattle and humans. For instance, infertility is a costly issue for the beef industry, representing a $4.7 billion annual loss for U.S. cattle producers.”

“Eventually this could also lead to better fertility diagnostics for humans,” he said. “One out of eight couples struggle with infertility. We know that men contribute up to two-thirds of the problem directly and indirectly. Current human infertility treatments are not only very expensive, with low success rates, but also can put undue stress on the emotional health of the couple when not diagnosed correctly. Having better diagnostics to identify male fertility issues, and better ways to address them, could reduce the stigma we now often unduly place on women and greatly boost reproductive success for couples.”

Read more from Farm Journal’s PORK:

Boar Fertility: It’s Not Just About Form and Speed

Hogs Reproduction

Iowa State Livestock Research Aims to Uncover How Biological Factors Impact Human Fertility

How Does Iowa State Research on Swine Sire Fertility Impact Cattle, Humans?